Midranger 1kb dna ladder 300bp 5, 000bp catalogue number. This 1kb dna ladder is a convenient marker for determining the size of doublestranded dna from 250 to 10,000 base pairs. Feb 15, 2012 in a 1 kb ladder, the bands are 1kb apart in size, though ones at the lower end may be closer in size than 1kb. Unless otherwise indicated, 200 nm of each strand was assembled in 0. The 1 kb plus dna ladder is suitable for sizing linear doublestranded dna fragments from 100 bp to 12 kb. William smith general phone 2755731 molecular biology sales specialist. How can i prepare working solution with 1 kb dna ladder. The 1 kb plus dna ladder ranges from 100bp 10kb and and is supplied with a. Dna ladder 100 bp and dna ladder 1kb marker for dna rna agarose gel electrophoresis. Generuler 1 kb dna ladder thermo fisher scientific. This product is covered by one or more patents, trademarks andor s owned or controlled by new england biolabs, inc neb. Nucleic acid ladder is used as a size standard in agarose gel electrophoresis. The 1 kb dna ladder, containing linear doublestranded dna fragments, is suitable for sizing doublestranded dna from 250 bp to 10,000 bp.
One kb plus dna ladder is composed of 20 highly purified, doublestranded dna bands spanning 100bp to 12,000bp. Thermo scientific ogeneruler 1 kb dna ladder, readytouse 25010,000 bp perform sizing and approximate quantification of a wide range of doublestranded dna on agarose gels with this 1 kb dna ladder. Thermo scientific generuler 1 kb dna ladder, readytouse, is recommended for sizing and approximate quantification of a wide range of doublestranded dna on agarose gels. Generuler 1 kb plus dna ladder catalog number sm31, sm32 pub. Pdf dna ladder is commonly used to determine the size of dna fragments by electrophoresis in. Thermo scientific generuler 1 kb plus dna ladder is recommended for sizing and approximate quantification of a doublestranded dna in the range of 75 bp to 20,000 bp on agarose gels. This ladder consists of bands containing 18 repeats of a 1018bp dna fragment.
Ready to load ladders contain highly purified pcr products combined with plasmid digests, they are supplied in 1x loading dye. Dna ladders have defined sizes, and are not intended for use in quantitative analysis. Sufficient plasmid dna can be isolated from 100 ml e. Two readytouse dna size markers for gel electrophoresis, the 100 bp ladder and 1 kb ladder represent the two most popular markers used for dna fragment size determination. A dna ladder is a solution of dna molecules of different. This marker can be used with either agarose or polyacrylamide gels. A number of proprietary plasmids are digested to completion with appropriate restriction enzymes to yield 10 bands suitable for use as molecular weight standards for agarose gel electrophoresis. If the ionic strength of the sample is too low, blurring of the bands can occur. Generuler 1kb dna ladder, range 250 to 10,000bpcan be labeled radioactively with t4 polynucleotide kinase seeprotocol. The apparent molecular weight of each protein kda has been determined by calibration against an unstained protein ladder. Generuler 1 kb dna ladder, readytouse thermo fisher scientific. For quantification, adjust the concentration of the sample to equalize it approximately with the amount of dna in the nearest band of the ladder. Dna fragments of 100 bp with unique restriction site at both ends were self ligated to create a tandem repeat.
Please have a look into the related application note for more information about ladder migration in different gel concentrations. Highranger 1kb dna ladder 300bp 10,000bp catalogue number. The 1 kb and 3 kb bands contain more dna to provide internal orientation. Thermo scientific generuler 1 kb dna ladder, readytouse. Contents amount storage sm03 generuler 1 kb dna ladder, readytouse 250 5 x 50 g for 500 applications, 0. Hyperladder 1kb is our most popular molecular weight marker, composed of a restriction digest plus one or more pcr products, especially designed for easy. Load the same volumes of the dna sample and the dna ladder. For optimal resolution, the recommended agarose gel concentration is 0. Trackit 1 kb plus dna ladder thermo fisher scientific. The ladder is composed of fourteen chromatographypurified. Thermo scientific generuler 1kb dna ladder, readyto use, is designed for sizing and approximate quantification of.
The 1 kb dna ladder contains discrete dna fragments ranging in size from 300 bp to 10,000 bp. The ladder is supplied with 6x dna loading dye, and it can be used in dna sizing and approximate quanitification applications. Use the loading dye solutions supplied with every fermentas dna laddermarker, as these solutions contain equilibrated amount of tracking dyes which will not mask dna under uv light. Thermo scientific generuler 1 kb dna ladder is designed for sizing and approximate quantification of wide range doublestranded dna on. Pdf creator software latest version free download 16rqrz pdf creator software latest version free download 3d39b66ab9. Pdf straightforward procedure for laboratory production of dna. Invitrogen trackit 1 kb plus dna ladder is ready to use and designed for sizing and approximate quantification of doublestranded dna in the range of 100 bp to 15,000 bp. The ladder consists of fragments ranging in size from 250 to 10,000 base pairs. The digested dna includes fragments ranging from 0. Generuler 1 kb dna ladder 1250 25 x 50 g for 2500 applications, 0.
Thermo scientific generuler 1 kb dna ladder is recommended for sizing and approximate quantification of a doublestranded dna in the range of 250 bp to 10,000 bp on agarose gels. The concentration of 1kb dna ladder is 100 ugml 500ul five microlitres 500 ng of the 1 kb dna ladder are mixed. The ladder is composed of fifteen chromatographypurified. The dna ladder consists of proprietary plasmids which are digested to completion with appropriate restrict. Goldbios readytouse 1kb ladder serves as a clear dna standard with band fragments and greater band intensity for reference at the 3kb and.
Hyperladder 1kb with 1 % wv agarose gel, hyperladder 50bp with 1. This marker is ideal for the size determination of digested dna. While neb develops and validates its products for various applications, the use of this product may require the buyer to obtain additional third party intellectual property rights for certain applications. This visual guide allows you to easily compare the differences between dna ladders offered at goldbio in terms of size. Use the loading dye solutions supplied with every fermentas dna ladder. U of rochester biological supply ctr g8633 stock manager. Sigmas 1kb ladder is provided in a solution of 10 mm trishcl ph 8. The 1 kb dna ladder is a unique combination of a number of proprietary plasmids digested with appropriate restriction enzymes and pcr products to yield fragments, suitable for use as molecular weight standards for electrophoresis. Quickload 1 kb dna ladder is a premixed, readytoload molecular weight marker containing bromophenol blue as a tracking dye. Generuler 1 kb plus dna ladder thermo fisher scientific. Thermo scientific generuler 1 kb plus dna ladder is designed for sizing and quantification of dna fragments in agarose gels. Supplementary information 1 for isothermal self assembly of. The product is supplied in a solution of 10 mm trishcl, ph 8.
The ladder is premixed with gel load buffer color dyes with. Dna ladder 100 bp 1kb marker for agarose gel electrophoresis. The dna ladder consists of proprietary plasmids which. Product information thermo scientific generuler 1 kb dna.
Generuler 1 kb plus dna ladder 1250 25 x 50 g for 2500 applications, 0. Phylogenetic analysis and prey identification of spiders from. The apparent molecular weight of each protein kda has been determined by calibration against an unstained protein ladder in each electrophoresis conditions. Thermo scientific ogeneruler 1 kb dna ladder, readyto. Description thermo scientific generuler 1 kb dna ladder is. Do not heat the 1kb plus dna ladder before loading. Use the 1 kb dna extension ladder to size linear doublestranded dna fragments from 500 bp40 kb. View more information about our dna stains technology. The dna ladder consists of 14 dna fragments and is provided with 6x tritrack dna loading dye. The dna includes fragments ranging from 25010,000 base pairs.
Material safety data sheet full scale dna ladder, 1kb. The 1 kb dna ladder was not designed for precise quantification of dna mass, but can be used for approximating the mass of dna in comparably intense samples of similar size. Page 1 4 material safety data sheet prepared in accordance with eu directive 91155eec printed. You really need to check the technical info that comes with the ladder for the details on each product, as some have different product names which mean the same thing. This product is available in quickload purple format enjoy the convenience of a readytoload format. Contents amount storage sm0311 generuler 1 kb dna ladder 250 5 x 50 g for 500 applications, 0. The dna ladder consists of 15 dna fragments and is provided with 6x tritrack dna loading dye. The following file naming structure is used to name the majority of these document files.
The ladder is a mixture of chromatographypurified individual dna fragments. Generuler 1 kb dna ladder, readytouse catalog number sm03, sm0314 pub. The dna 1 kb ladder is suitable for size determination of doublestranded dna using dna electrophoresis. Straightforward procedure for laboratory production of dna ladder. The 1 kb dna ladder was not designed for precise quantification of dna mass, but can be. How can i prepare working solution with 1 kb dna ladder supplied by promega. If samples need to be diluted, use te or other buffer of minimal ionic strength. Quality control tests are performed on each new lot of neb product to meet the specifications designated for it. Thermo scientific generuler 100bp dna ladder 100bp, 0. The 1kb dna ladder has thirteen bluntended fragments with sizes ranging from 250bp to 10,000bp. The ladder consists of bands that are generated from pcr and restriction enzyme digestion of double stranded dna. Pdf straightforward procedure for laboratory production.
Generuler dna ladders are ideal for dna sizing and approximate quantification of a wide range of doublestranded dna ranging from 10 bp to 48. Do not exceed the amount of electrophoresis tracking dyes used for sample ladder preparation. Generuler 1 kb dna ladder catalog number sm0311, sm0312 pub. For your convieniance there is a picture of the ladder presented here, but you should go to the fermentas site to read up on this ladder. By selecting the generuler dna ladder of interest in table 1, you will be directed to the corresponding product page for more details and ordering information.
Product information thermo scientific generuler 1 kb. Please have a look into the related application note for more information about ladder. Two readytouse dna size markers for gel electrophoresis, the 100 bp ladder and 1 kb ladder. High quality readytouse doublestranded dna ladder derived by recombinant.
A unique combination of a number of proprietary plasmids digested with appropriate restriction enzymes and pcr products to yield fragments, suitable for use as molecular weight standards for agarose gel electrophoresis. The 1,000bp and 3,000bp fragments have increased intensity relative to the other bands on ethidium bromidestained agarose gels for easy identification. The 1636 bp band and all bands less than bp are generated from pbr322. The ladder is composed of fifteen chromatographypurified individual dna. Pageruler prestained protein ladder 10 170 kda migration patterns were determined using respective nupage precast gels. Do not exceed the amount of electrophoresis tracking dyes used for sampleladder preparation. Thermo scientific generuler 1 kb dna ladder 1kb dna ladder. The ladder is premixed with gel load buffer color dyes with varying mobility on an agarose gel for direct transfer to the gel.